Generator

Part:BBa_K323001:Design

Designed by: Jernej Turn�ek   Group: iGEM10_Slovenia   (2010-10-20)

cYFP link Zif268 under T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 790
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 790
    Illegal NotI site found at 796
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 790
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XbaI site is present instead of scar after His tag. Scar is shown since BioBricks cannot include BioBrick restriction sites.

Source

References